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Farghaly et al. Hepatoma Res 2018;4:41 I http://dx.doi.org/10.20517/2394-5079.2018.30 Page 7 of 10

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Figure 3. Down-regulation of L22 in Alu-RNA element dependent manner. (A) Relative expression of Alu non-coding gene in cells that
were treated with heat shock (HS) in comparison with non-treated cells (control) using qRT-PCR; (B) relative expression of L22 gene in
cells that were treated with HS in comparison with non-treated cells (control) using qRT-PCR. Error bars indicate the standard deviation
of two independent experiments; (C) the possible binding site and seeding region of Alu repeated sequences (query) and RPL22
sequences (target) indicated by IntaRNA software

Figure 4. Interruption of L22 corresponding protein in response to heat shock (HS). The expression protein level of RPL22 elevated
by percentage of positive HepG2 cells that were treated with HS and infected with HCV compared to control infected cells using
flowcytometry