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Extracell Vesicles Circ Nucleic Acids 2020;1:20-56 I http://dx.doi.org/10.20517/evcna.2020.10 Page 53

tissue infection sites including the lungs and GI system, and can lead to a CNS Parkinsonian phenotype.
With evidence growing for neurotropism of the SARS-CoV-2 coronavirus and potentially for many other
emerging viruses, it is important to determine the precise mechanisms by which infectious agents and EMVs
[9]
may contribute to latent disease, including neurodegenerative diseases and cancers that may arise later in life .

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Rep 2017;7:11496.

44. Tracking exosomes in vitro and in vivo

1,3,4
1,2
3
2
Authors: Frederik J. Verweij , Maarten Bebelman , Graça Raposo , D. Michiel Pegtel , Guillaume van
Niel 1
E-mail:
FJV: frederik.verweij@inserm.fr;
DMP: d.pegtel@amsterdamumc.nl;
GVN: guillaume.van-niel@inserm.fr
Affiliations:
1 Institute for Psychiatry and Neurosciences of Paris, Hopital Saint-Anne, Université de Paris, INSERM U1266,
Paris, France.
2 Institut Curie, PSL Research University, CNRS, UMR144, Paris, France.
3 Department of Pathology, Cancer Center Amsterdam, Amsterdam University Medical Center, Amsterdam, The
Netherlands.
4 Division of Medicinal Chemistry, Amsterdam Institute for Molecules Medicines and Systems, VU University,
Amsterdam, The Netherlands.

Abstracts: Exosomes are a subclass of Extracellular Vesicles able to elicit a phenotypic response in target
cells, and correspond to the intraluminal vesicles (ILVs) of multivesicular endosomes (MVEs) formed
during endosome maturation. Before these MVEs fuse with the plasma membrane - the final step of
exosome biogenesis - they undergo dynamic interactions with other endosome-subtypes and organelles
that will impact the dynamics of exosome secretion. After secretion, they face the complex 3D environment
of the body, before being taken up by target cells. Due to a lack of appropriate model systems, it remains
challenging to understand how factors impact their physiology in vivo. We developed in vitro and in vivo