Page 26 - Read Online

Page 22 Extracell Vesicles Circ Nucleic Acids 2020;1:20-56 I http://dx.doi.org/10.20517/evcna.2020.10

No. Abstract Title Authors Pages
31 Mycobacterial dynamin like proteins are necessary for Shamba Gupta, Ainhoa Palacios, Atul Khataokar, 43
extracellular vesicle release in M. tuberculosis Madhuri Bhagavathula, Matthew B. Neiditch, Padmini
Salgame, Rafael Prados-Rosales,
G. Marcela Rodríguez
32 Intracellular delivery methods using biofunctional peptide- Ikuhiko Nakase 44
modified extracellular vesicles
33 Effects of marijuana on viral transcription in HIV-1 infected Catherine De Marino, Maria Cowen, Bianca Cotto, Sara 45
cells and resulting extracellular vesicle release Jane Ward, Ronald Tuma, Prasun Datta, Leah H. Rubin,
Dianne Langford, Fatah Kashanchi
34 Extracellular vesicles release from infected cells prior to Yuriy Kim, Daniel Pinto, Gifty Mensah, Maria Cowen, 45
virion release James Erickson, Renaud Mahieux, Fatah Kashanchi
35 Separation of EVs from virions in coronavirus infections James Erickson, Maria Cowen, Yuriy Kim, Anoop K. Pal, 46
Heather Branscome, Archana Gupta, Fatah Kashanchi
36 Reduction of the therapeutic dose of silencing RNA by Ryan Reshke, James A. Taylor, Alexandre Savard, Huishan 47
packaging it in extracellular vesicles via a pre-microRNA Guo, Luke H. Rhym, Piotr S. Kowalski, My Tran Trung,
backbone Charles Campbell,Daniel G. Anderson, Derrick Gibbings
37 Cell type-specific and disease-associated protein networks Yang You, Satoshi Muraoka, Mark P. Jedrychowski, 48
in extracellular vesicles isolated from human iPSC-derived Jianqiao Hu, Amanda K. McQuade, Tracy Young-Pearse,
neural cells and Alzheimer’s disease brain tissues Roshanak Aslebagh, Scott A. Shaffer, Mathew Blurton-
Jones, Wayne W. Poon, Steven P. Gygi,
Tsuneya Ikezu
38 Exosomes mediate Zika virus transmission through SMPD3 Hameeda Sultana 49
neutral Sphingomyelinase in cortical neurons
39 Understanding Intracellular Fate of EV-delivered Content Killian P. O’Brien, Stefano Ughetto, Xandra O. Breakefield 49
40 Smart Microprobes Imbued with Recognition Element as a Chukwumaobim D. Nwokwu, Saif Mohammad Ishraq 50
Sensitive Bioanalysis Platform for Exosomes Bari, Gergana G. Nestorova
41 Extracellular vesicles for drug delivery to the brain Bhagyashree S. Joshi, Marit A. De Beer, Ben N.G. 50
Giepmans, Sameh A. Youssef, Alain de Bruin, Harm H.
Kampinga, Inge S. Zuhorn
42 The uptake, trafficking, and biodistribution of Bacteroides Simon Carding 51
thetaiotaomicron (Bt) generated outer membrane vesicles
43 Infectious Exosomes/Microvesicles in Degenerative and Dennis A. Steindler 52
Neoplastic Stem Cell Pathologies
44 Tracking exosomes in vitro and in vivo Frederik J. Verweij, Maarten Bebelman, Graça Raposo, D. 53
Michiel Pegtel, Guillaume van Niel
45 A CRISPR-Cas9-based reporter system for single-cell Olivier G. de Jong, Daniel E. Murphy, Imre Mäger, Eduard 54
detection of extracellular vesicle mediated functional Willms, Antonio GarciaGuerra, Jerney J. Gitz-Francois,
transfer of RNA Juliet Lefferts, Dhanu Gupta, Sander C. Steenbeek,
Jacco van Rheenen, Samir El Andaloussi, Raymond M.
Schiffelers, Matthew J. A. Wood, Pieter Vader
46 Exploitation of the Leishmania Exosomal Pathway by Martin Olivier 54
Leishmania RNA virus 1
47 Delivery of pre-miRNA Cargo to Tumor Microvesicles Crislyn D’Souza-Schorey, James Clancy, Ye Zhang 55
48 Head and Neck Cancer Exosomes Drive MicroRNA- Patrick J. Hunt, Moran Amit 56
mediated Reprogramming of Local Neurons

1. Argonautes in extracellular vesicles: artifact or selected cargo?

Author: Alissa M. Weaver
E-mail: alissa.weaver@vanderbilt.edu
Affiliations: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine,
Nashville, TN, USA.
Abstracts: Argonaute 2 (Ago2) is the essential component of the RNA-Induced Silencing Complex (RISC)
that binds miRNAs and promotes mRNA degradation. Extracellular vesicle (EV)-carried miRNAs have
been shown to influence gene expression and functional phenotypes in recipient cells. Many investigators
have found Ago2 in EVs and it is postulated that Ago2 is a major transporter of miRNAs into small EVs
(SEVs), such as exosomes. Others have reported that extracellular Ago2 is non- vesicular. I will discuss
issues that may lead to diverging results and show data on the role of culture conditions in the detection of
Ago2 in SEVs.